nm4bl2103 – Continous monitoring of cell transfection efficiency on micropatterned substrates

POSTER_BCMATERIALS_EAH_2021
Continous monitoring of cell transfection efficiency on micropatterned substrates

Conventional cell cultures lack control over cell interactions and usually require detachment of the cells for the quantification of gene transfection efficiency. In order to study the effect of cell-cell contact on the efficiency of transfection, we applied micropatterning of mesenchymal stromal cells to generate two different cell-cell contact scenarios, one of single cells and one of small cell-colonies, Figure 1, through microcontact printing in order to study the effect of cell-cell contact on the transfection efficiency.